Clinton Scandal Essays (452 words) - Lewinsky Scandal, Linda Tripp

Clinton Scandal Let American Consumer Counseling Help you Get Out of Debt! Clinton Scandal The evidence against President Clinton for sexual misconduct is very strong. There have been too many scandals that have surrounded his presidency. This forces the citizens of the United States to deny his plea of innocence. In the spring of 1995, Monica Lewinsky graduated from Lewis and Clark College. She then started an unpaid internship at the White House. Allegedly in November of that year, she became involved in a sexual relationship with President Clinton. Shortly after this point Lewinsky was hired as a full-time White House employee. She left the White House for a public affairs position at the Pentagon. Here she became friends with Linda Tripp, a former White House employee, who worked in the same office. During this time President Clinton apparently sent gifts to Lewinsky, most importantly in the case against him a dress, which she saved as a souvenir, had the President's DNA on it. With things heating up in the Paula Jones case, the President asked Lewinsky to lie about visits to his office. Turning her back on Lewinsky, Tripp taped telephone conversations between herself and Lewinsky about the affairs with Mr. Clinton to setup him up. Lewinsky, allegedly quoting Mr. Clinton, said, "Look them in the eye and deny it." After anonymous tips are given to a firm connected with the Paula Jones case, Lewinsky decided to leave the government and find a new job. On January 7, 1998, Monica Lewinsky signed a sworn affidavit in the Paula Jones sexual harassment case, stating she did not have an affair with the President. Following this, Linda Tripp brought the recorded phone conversations to Independent Counsel for the Paula Jones case, Kenneth Starr. With assistance from the FBI, Tripp wore a wire while Lewinsky lead her step-by-step through her entire sexual relationship with Mr. Clinton. The following day, Lewinsky gives Tripp points on how to respond to Paula Jones' lawyer's questions. After receiving news of this Starr requested from a federal panel, the ability of an expansion of his ongoing investigation. The following day, President Clinton, who was under oath, denied an affair with Lewinsky. Four days later, the story of the tapes became public knowledge. The President stated in an interview with Jim Lehrer of PBS on the same day, "There is no improper relationship"(Clinton 1998). He also stated in this interview that he "didn't ask anybody not to tell the truth."(Clinton 1998). In using a double negative, Mr. Clinton could possibly be telling the people of the United States that he did have an affair, without expanding on it. With all of the allegations against the President seeming to the public to be against his favor, he will be found guilty and should be impeached. If this is a conspiracy against him, the conspirator or co-conspirators will face major indictments.

Supply and Elasticity

Supply and Elasticity Define Supply and explain what Causes Change (shifts) of Supply and how Supply Determines Prices Supply refers to the ability and readiness of sellers to create and offer different quantities of a commodity at different prices, or during a certain phase (Arnold 2010). Similar to demand, supply can also change. The supply of a commodity can increase or go down. Supply increases when suppliers can produce and are ready to sell the good at each price.Advertising We will write a custom essay sample on Supply and Elasticity specifically for you for only $16.05 $11/page Learn More For instance, sellers could produce and be ready to sell 50 dresses at $20 each in May, while in June they could have the capacity to produce and be willing to sell 70 dresses at $20 each. If supply increases, the entire supply curve shifts to the right as shown in fig (a). Fig (a) Shifts when Supply Increases On the other hand, supply decreases when sellers decide to produce and sell l ess of a good at all prices. For instance, sellers can produce and be willing to sell 50 dresses at $20 each in May, while in June, they could have the capacity to produce and be willing to sell 25 dresses at $20 each. If supply decreases, the entire supply curve shifts to the left as shown in fig (b). Fig (b) Shifts when Supply DecreasesAdvertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Many factors affect the supply of goods and interfere with supply curves. These factors include prices of other commodities, technology, prices of related resources, prospects of future price, the amount of sellers, government restrictions and taxes (Arnold 2010). Price of Related Resources Resources are central in the manufacture of goods. For instance, manufacture of tables needs wood. Table production becomes less expensive when the price of wood decreases. The profits from manufacturing tables increase as price of tables remains the same even ass cost reduces. Consequently, the economic incentive to manufacture more tables boosts. Table manufacturers will make and sell an increased number of tables at each price. Therefore, the supply curve of tables will rise and this curve will shift to the right direction. Manufacture of tables becomes more expensive when the price of wood increases. As a result, the supply of tables will fall and the supply curve of tables will shift to the left direction. Prices of other Goods Changes in price of a single commodity affect the supply of other goods. For instance, a company that manufactures plastic cups may start manufacturing glass cups, when the price of glass cups rise. Quantity of Sellers When an increased number of sellers begin selling a commodity due to high profits the supply curve moves to the right. When the number of sellers reduces due to losses the curve shifts to the left. Technology Technology refers to expertise in use of resources during production (Arnold 2010). An advance in technology refers to the capacity to yield increased output with a fixed measure of resources and this lessens costs in unit production. For instance, lest assume that it takes $80 to create 20 units of a product. The cost of production per unit is $4. Suppose an advance technology can produce 40 units at a cost of $80, the cost of production falls to $2, for every unit. When the per-unit cost of production falls, quantity of supply increases.Advertising We will write a custom essay sample on Supply and Elasticity specifically for you for only $16.05 $11/page Learn More Reduced costs of each unit boost profitability and these acts as an incentive to manufacturers to create more products. For instance, if farmers discover a way to grow peas using the same quantity of resources, cost of production per unit decreases, profits rise and farmers get the incentive to grow and sell more peas at each price . The curve of supply here shifts to the right. Future Price Expectations Producers may stop producing a commodity if they predict that it will sell at a higher price in prospect. In this situation, the supply curve will shift to the left. For instance, if wheat producers expect that the prices of wheat will rise next year, they may stop selling wheat pending next year. Equally, if wheat producers expect that the price of wheat will fall next year, they will sell more wheat this year. Government Restrictions At times, the government may decide to cut supply through imposing an import quota. For instance, a U.S. import quota on Chinese mobile phones will cut the supply of China mobile phones in America and the curve will shift to the left. Similarly, the supply of Chinese mobile phones increases upon removal of the import quota and the supply curve shifts to the right. Taxes and Subsidies Taxes increase the costs of commodities. When the rates of taxes rise, producers tend to produce less commodities and the curve shifts to the left. Besides, when the rates of taxes decrease, producers tend to produce more goods and the supply curve turns to the right. Explain what is Price Elasticity of Supply and its Determinants and why does it Matter whether Supply is Price Elastic, Inelastic or Unit-Elastic? According to the law of supply, higher prices increase the amount of supply. The price elasticity of supply gauges how the amount of supply changes with price (Mankiw 1998). The supply of a product becomes elastic when the amount supplies responds to pr ice change significantly. Supply becomes inelastic when the amount supplied reacts to price changes insignificantly. The flexibility of sellers to change the quantity of good they produce affects price elasticity. For instance, land at the beach is inelastic, since production of more land is impossible. On the other hand, commodities like cups, seats and radios have elastic supplies, since companies that produce them ca n extend the time for work in response to a higher price. The main determinant of the price elasticity of supply in markets is the phase of time under consideration. Supply becomes more elastic after an extensive period than after a short duration (Mankiw 1998). Companies cannot change their quantity of production over a short phase.Advertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Therefore, the amount supplied in the short run does not change in reaction to changes in price. On the other hand, companies may create new plants, or close old ones in the long-term. Besides, new companies can penetrate a market as the old ones end. Therefore, the amount supplied can react to price significantly, in the long-term. Price elasticity of supply matters because it helps in determining how price is reacting to changes in demand. For instance, coffee demand may change due to introduction of substitutes at a lower price, or due to people’s sensitization about the negative impact of caffeine in coffee. When the price elasticity of supply is high, the price changes insignificantly compared to when the price elasticity of supply is low. A slight change in price can make companies to cut the quantity supplied down to the lower quantity demand, with high price elasticity (Taylor 2006). Therefore, the elasticity of supply affects the equilibrium price and change in quant ity. After considering supply elasticity, we can foresee what happens to prices following a shift in the supply of a commodity. We can as well forecast what the impact of a change in demand on price. References Arnold, R 2010, Microeconomics, Cengage Learning, London. Mankiw, G 1998, Microeconomics, Elsevier, New York. Taylor, J 2006, Principles of macroeconomics, Cengage Learning, London.

Greenleaf by Flannery O'Connor Essay Example | Topics and Well Written Essays - 500 words

Greenleaf by Flannery O'Connor - Essay Example She is inwardly tormented by a world that is not of her making and outwardly by a wild bull that is eating away at what little peace she has left on earth. The Greenleaf sons were content with their modern dairy operation while Mr. Greenleaf and his wife, "... lived like the lilies of the field, off the fat that she struggled to put into the land". "Greenleaf" is a story of families and faith. . Mrs. May had hired the Greenleafs after her husband passed away and left the dairy farm to her and her two sons. When they were hired, Mr. Greenleaf and his two sons brought along Mrs. Greenleaf and 5 little girls. At this point in their history the families were not remarkably different. Mr. Greenfield's total possessions were little more than a "... pieced-together truck, his wife and five daughters". Mrs. May had inherited little more than that from her husband. Her friends were awestruck that she had gone "...practically penniless and with no experience, out to a rundown farm...". Still, Mrs. May viewed herself as a remarkable woman. The hired help were a necessary annoyance. The sons also grew in different directions. The Greenleaf boys, O.T. and E.T., spent a successful stint in the Army and returned with their new wives to set up a successful dairy business. Tormented by her own sons' failures, Mrs. May attributed E.T.'s and O.T.'s success to government handouts.

An Exploration into the Role of Nutrition Information in Influencing Literature review

An Exploration into the Role of Nutrition Information in Influencing Consumer Choice - Literature review Example The strong association between nutrition and health, alongside the ever rising problem of obesity justify a study into nutrition consumerism. The main aim of this literature review is to explore the role of nutrition information in influencing consumer choices. Objectives The following objectives will guide this review of literature towards achieving the stated aim: To perform a prudent search for relevant literature detailing the role of nutritional information on consumer choices To review the literature noting highlights and emerging themes To arrive at a conclusion based on the literature review on the role of nutritional information on consumer choices Literature Review The approach followed for sourcing the literature involved a search for research and theoretical work through the key phrase â€Å"Role of Nutrition Information in influencing Consumer Choice.† The search was focused on a number of peer-reviewed journals for the research articles. Further, a look at the bi bliographies of the journals was used to find more relevant literature. The sources selected for use were restricted to the last decade in order to ensure this literature review is up-to-date, valid and relevant. ... Factors determining Consumer Choices in Nutritional Purchases A number of studies have explored the factors determining consumer choices when making food purchases. One of these is by Petrovichi and Ritson (2006) who investigated the factors that influence individual decisions to engage in dietary health preventative behaviour in Romania. Conceptually, the close relationship between health and nutrition translates to an association of managing nutrition practices to prevent or manage health. The methodology adopted by the two researchers entailed face-to-face issuance of questionnaires to 485 adult respondents. This study established that the respondents’ health motivations, beliefs that diet choices have the capacity to prevent diseases, financial status of households and the level of educational influenced their nutrition and health and thus food purchase decisions. Ling and Horwath (2001) established that the perceived benefits of certain dietary products influenced consume r food purchase decisions. Through a cross-sectional telephone and mail survey, the scholars gathered data from 1200 households. They showed that the perceived benefits of fruits and vegetables in diet tipped the decisional balance towards purchasing these food items. Godwin, Speller-Henderson and Thompson (2006) undertook a consumer survey involving 160 participants and established that knowledge about nutrition greatly influenced consumer preferences in food purchases; the majority of respondents perceived themselves as knowledgeable enough to understand nutrition labels and use these to make purchase decisions. To conclude this part, the general factors that influence consumer purchase decisions include knowledge on nutrition; health

Critically discuss whether it is important for children to feel that Essay

Critically discuss whether it is important for children to feel that they 'fit in'. How can practitioners support the development of a sense of belonging - Essay Example Fitting in has come to take on a negative tone where it is believed that children are forced to adopt the mannerisms of the group that they wish to join in order to feel accepted. Not only does it create a situation where children end up losing themselves, but it makes these individuals live a lie, where they pretend to behave in a certain manner so that they can be able to achieve popularity in their environment, especially in school. This paper seeks to show that despite fitting in being considered important, it is detrimental to the development of children because it ends up not giving them an opportunity to belong. In most cases, children seek to fit in so that they can be considered by their peers to be popular and this is especially the case in schools. Schools tend to bring large groups of children together in a manner that creates a situation where there develop a diverse number of social networks among them (Faircloth and Hamm, 2011). These social networks are vital for children within these institutions because they assume the role of socialising them as well as ensuring that they are able to have a circle of friends on which they can rely. However, these social networks tend to be extremely hierarchical and this in such a manner that the most popular children end up being considered to be at the top of the hierarchy while the least popular end up at the bottom. This creates a situation where there is constant competition among children from an early age to ensure that they are able to join the popular children and through them to also gain popularity. While it may seem to be a commendab le system because it gives children the opportunity to learn the means through which they can be able to survive in a competitive world as adults, it also brings about problems for these children (Cialdini and Goldstein, 2004). This is especially the case where children end up having to reject their own personalities and adopt the behaviours of the group that

Identification of Plant Material: Corallocarpus Epigaeus L

Identification of Plant Material: Corallocarpus Epigaeus L 6. MATERIALS AND METHODS 6.1 Collection The plant material was collected from the Tirupati, Andhra pradesh, India in the month of February 2014. The plant taxonomical authenticated by Prof; Dr. K. Madhava chetty, Department of Botany, SV University, Tirupati. The voucher specimen of Corallocarpus epigaeus L has been preserved in our laboratory for further collection and reference. Chemicals Various reagents like Mayer’s reagent, Wagner’s reagent, Hager’s reagent, Dragendroff’s reagent, ÃŽ ± napthol solution, Fehlings solution A B, Barfoeds reagent, Millons reagent, Ninhydrins solution, cuso4, ethanol 95%, potassium hydroxide, concentrated HNO3, pyridine, sodium nitroprusside, sodium picrate, concentrated HNO3, pyridine, sodium nitroprusside, sodium picrate, concentrated H2SO4, Glacial acetic acid, Ferric chloride, Ammonium hydroxide solution, Potassium dichromate solution, Thionyl chloride solution, Phenolpthalein, Chloroform, etc. were received from standard suppliers to Dept of Pharmacology, SIPS-Proddatur. 6.1.1 Preparation of Whole Plant ethanolic extract of Corallocarpus epigaeus L The fresh leaves of Corallocarpus epigaeus L. The sieved powder was stored in airtight container and kept at room temperature for further study. The dried powdered material (250gm) was extracted with 95% ethanol using soxhlet apparatus for about 72hours. Figure no 12: SOXHLET APPARATUS DISTILLATION APPARATUS After extraction with solvent, the marc was dried in hot air oven below 50o c and was concentrated by distilling off the solvent and evaporating to dryness. The dried extract was subjected to preliminary phytochemical screening for detection of various phytoconstituents. 6.1.2 Qualitative Phytochemical Analysis74 The ethanolic extract Corallocarpus epigaeus L was subjected to various analytical tests in order to identify various phytoconstituents. Test for Alkaloids Mayer’s test To 1 ml of the extract, a drop or two drop of Mayer’s reagent was added by the side of test tube. Appearance of a white or creamy precipitate indicates presence of alkaloids. Wagner’s Test To 1 ml of the extract, few drops of Wagner’s reagent was added. Development of reddish brown colour indicates the presence of alkaloids. Hager’s Test To the 1 ml of the extract, few drops of Hager’s reagent was added. A prominent yellow colour indicates the test as positive. Dragendroff’s Test To the 1 ml of the extract, few drop of Dragendroff’s reagent was added. A prominent yellow colour indicates the test as positive. Test for Carbohydrates Benedict’s Test To 5 ml of Benedict’s reagent, 1 ml of the extract solution was added and boiled for 2 minute and cooled. Formation of red precipitate shows the presence of carbohydrates. Molisch’s Test To 2 ml of extract, two drops of alcoholic solution of ÃŽ ±-naphthol was added and shaken well. Later 1 ml of concentrated sulphuric acid was added slowly along the side of the test tubes and allowed to stand. A violet ring indicates the presence of carbohydrates. Fehling’s Test To 1 ml of the extract, add equal quantity of Fehling solution A and B were added. Appearance of red precipitate indicates the presence of sugars. Barfoed’s Test To 2 ml of the extract, 2 ml of Barfoed reagent was added and mixed well. It was heated for 1-2 minute in boiling water bath and cooled. Formation of red precipitate Indicates the presence of sugars. Test for Protein and Amino Acids Million’s Test To 2 ml of the extract, few drops of Million reagent was added. A white precipitate Indicates the presence of proteins. Ninhydrin Test To the 2 ml of the extract, two drops of Ninhydrin solution was added. A characteristic purple color indicates the presence of amino acids, proteins and peptides. Biurett Test To 1 ml of the extract, one or two drop of 1% copper sulphate solution was added and tothis 1 ml of ethanol (95%) was added, followed by excess of potassium hydroxide pellets. The pink layer in ethanolic layer indicates the presence of proteins. Xanthoprotein Test To 1 ml of the extract, add 1 ml of concentrated Nitric acid was added resulting in the formation of a white precipitate which is then boiled and cooled. Then 20% sodium hydroxide in ammonia was added. Orange colour indicates the presence of aromatic amino acids. Test for Glycosides Legal’s Test 2 ml of extract was dissolved in the solution of pyridine. Then sodium nitroprusside was added, to make it alkaline. The change in the colour from yellow to orange was not observed, which indicates the presence of glycosides. Baljet’s Test To 1 ml of the extract, 1 ml of sodium picrate solution was added. The colour from yellow to orange reveals the presence of glycosides. Borntrager’s Test To 1 ml of extract, few ml of sulphuric acid was added, boiled, filtered and extracted with chloroform. The chloroform layer was than treated with few ml of ammonia. The formation of red colour indicates the presence shows the presence of anthraquinone glycosides. Keller Killani Test The extract was dissolved in acetic acid containing traces of ferric chloride and was transferred to a test tube containing sulphuric acid. At the junction, the formation of reddish brown colour, which gradually turns to blue, confirms the presence of glycosides. Test for Flavonoids Shinoda Test To 1 ml of extract, magnesium turnings was added and 1-2 drops of concentrated hydrochloric acid was added drop wise. Formation of pink to crimson colour indicates the presence of flavonoids. Alkaline reagent Test The aqueous solution of the extract was treated with 10% ammonium hydroxide solution. Yellow fluorescence indicates the presence of flavonoids. Tests for Tannins and Phenolic compounds Ferric chloride Test To 1 ml of the extract, add few drops of neutral 5% ferric chloride solution. Formation of dark greenish colour shows the presence of phenolic compounds. To the extract add potassium dichromate solution, formation of a precipitate shows the presence of tannins and phenolic compounds. Test for Triterpenoids Two or three granules of tin metal were added to thionyl chloride solution present in a test tube. Later 1 ml of extract solution was added. The formation of pink colour indicates the presence of triterpenoids. Test for Saponins The 1 ml of the extract was diluted with distilled water and the volume was made up to 20 ml. The suspension was shaken in a graduated cylinder for 15 minutes. Appearance of foam indicates the presence of saponins. Tests for Fixed Oil Spot test A small quantity of the extract was pressed between two filter papers. Appearance of oily stain on the presence of fixed oils. Saponification Test A few drops of 0.5 N alcoholic potassium hydroxide solution was added to a small quantity of extract along with a drop of phenolphthalein. The mixture was heated on water bath for 2 hrs. Formation of the soap or partial neutralization of alkali indicates the presence of fixed oil. Tests for Steroids Libermann Buchard Test 1ml of the extract was dissolved in 2 ml of chloroform in a dry test tube. 10 drops of acetic anhydride and 2 drops of concentrated sulphuric acid was added to it. The solution turns to red, then blue and finally bluish green, indicating the presence of steroids. Salkowski Test The extract was dissolved in chloroform the extract in chloroform and equal volume of concentrated sulphuric acid was added. Formation of bluish red to cherry red colour in chloroform layer and green fluorescence observed acid indicates the presence of steroids. 6.2 INVITRO ANTICANCER ACTIVITY Cell Lines COLO 320 cell lineswere obtained from sugen Life Sciences Pvt. Ltd., Tirupati and cultured in RPMI 1640 medium (Difco, invitrogen corp, Canada). General information74 Organism Homo sapiens, human Tissue Colon Product Format Frozen Morphology Cells are rounded and refractile Culture Properties Loosely adherent, multicell aggregates Biosafety Level 1 Disease DukestypeC,colorectaladenocarcinomas Age 55 years Gender Female Ethnicity Caucasian Storage Condition Liquid nitrogen vapor phase GenesExpressed Serotonin,norepinephrine,epinephrine,Adreno Cortico Tropic Hormone (ACTH), parathyroid hormone. Tumorigenic effectsYes, in nude mice CommentsCells are weakly positive for keratins Culture Method The base medium for this cell line is formulated RPMI-1640 Medium. 6.2.1 Tryphan Blue dye Exclusion Assay Method75 Table 5: List of instruments Table 6: List of Chemicals Experimental Design The designed study consists of three groups viz: Negitive control, Control, Test. In the Negative control group the cell lines were incubated with the medium for a period of 24 hours. This group was designed to rule out the possibility of any growth inhibitory effect of certain compounds of medium. The control group was designed to rule out the effect of any residual or traces of solvent with which the extract was prepared on the growth inhibition of cell lines. Here the solvent employed was ethanol and hence it is added at the concentration of 0.1% (v/v) in distilled water. In test group different concentrations of test extract i.e, 10, 25, 50, 75 and 100 µg/ml are incubated with colo 320 cell lines for a period of 24 hours. This group was used to study the effect on cell line viability. Table7: Experimental Design to Study the Effect of Ethanolic Extract of Corallocarpus epigaeus L on colo 320 Cell Line Viability by Tryphan Blue Assay Procedure 1. An aliquot of cell suspension being tested for viability was centrifuged for 5 min andsupernatant was discarded. The size of the aliquot depends on the approximate number of cells present. The aliquot is taken such that it contained a convenient number of cells to count in a haemocytometer when suspended in 1 ml PBS and then diluted again by mixing with 0.4% Tryphan blue (e.g., 5 Ãâ€"105 cells/ml). 2. The cell pellet was resuspended in 1 ml PBS or serum free complete medium. Serum proteins stain with Tryphan blue and can produce misleading results. Hence determinations must be made in serum-free solution. 3. Mix 1 part of 0.4% Tryphan blue and 1 part cell suspension (dilution of cells) cells were mixed and allowed to incubate at room temperature for approximately 3 minutes, Cells were counted within 3 to 5 min of mixing with Tryphan blue, as longer incubation periods will lead to cell death and reduced viability counts. Mixing was performed in a well of a microtiter plate or a small plastic tube using 10 to20  µl each of cell suspension and Tryphan blue. 4. A drop of the Tryphan blue/cell mixture was applied on to haemocytometer. The haemocytometer on the stage of a binocular microscope and cells were focused clearly. 5. The no. of unstained (viable) and stained (nonviable) cells were counted separately in the haemocytometer. Seeding of Cells COLO 320 cells were cultured to reach the 80-90% confluency using RPMI 1640 medium. After reaching the desired confluency, culture was collected and centrifuged at 3000 rpm for 10 minutes to get cell pellet. The pellet was resuspended in 1ml of fresh culture media. Cell concentration was determined by Tryphan blue assay was performed by mixing 50  µlitres of culture and 50  µlitres of 0.4% tryphan blue dye. Finally cells seeded in 24 well plates at the concentration 10000 cells/ml and incubated at 5% co2 incubator at 370 c for 24 hours. Drug Treatment Cells were maintained in 24 well plates in triplicate for every concentration, and treated with different concentrations of (10, 25, 50, 75, 100 µgm). Corallocarpus epigaeus L, test compound and control groups were treated with medium and ethanol. The treated cells were incubated for 24 hours in 5% co2 incubator at 370c. Invitro Cytotoxic Assay After 24 hours incubation the cells were collected from each well in eppendroffs and centrifuged at 3000rpm for 10 min to get cell pellet, to the pellet 50 µlit of each medium and tryphan blue was added and mixed well to suspend the pellet. Cytotoxicity was screened by performing tryphan blue assay. Percent of growth inhibition was calculated by using the following formula 6.2.2 Micro Culture Tetrazolium Assay76 Plant Material Used: Whole plant ethanolic extract of Corallocarpus epigaeus L. Principle This assay is based on the capacity of mitochondria succinate dehydrogenase enzymes in living cells to reduce the yellow coloured watersolublesubstrate3(4,5dimethylthiazolyl)2,5diphenyltetrazoliumbromideintoaninsolublepurplecolouredformazanproductwhosecolouredismeasuredbymeansofELISAreaderat540nm.Onlyviablecellswithactivemitochondria reducesignificant amountsof MTT, since reduction of MTT can only occur in metabolically active cells. Figure 13: Reduction of MTT to a formazan compound by mitochondrial Enzymes Cell Lines Human colorectal adenocarcinoma- colo 320were obtained by sugen Life Sciences Pvt. Ltd., Tirupati from an authenticated supplier. Stock culture of these cell lines were cultured in RPMI -1640 with 10% inactivated newborn bovine serum, Penicillin (100 IU/ml), Streptomycin (100 µg/ml)) under humidified. The cells were dissociated in 0.2% trypsin and 0.02% EDTA in phosphate buffered saline solution. The stock culture was grown in 25cm 2 tissue culture flasks and cytotoxicity experiments were carried out in 96 well microtiter plates. Procedure Cell lines in the exponential growth phase were selected, washed, trypsinized and suspended in complete culture media i.e, RPMI 1640. The microtiter plates and incubated for 24hrs during which a partial monolayer was formed. They were then exposed to various concentrations of the extract (1-100 µg/ml). Control wells received only the maintenance medium. The plates were incubated at 37 °C and 48 hrs and cells were periodically checked for granularity, shrinkage and swelling. After 48 hrs, the sample solution in wells was flicked off and 50 µl of MTT dye was added to each well. The plates were gently shaken and incubated for 4 hrs at 370C in 5%CO2 incubator. The supernatant was removed and 50  µl of DMSO was added. The plates were gently shaken to solubilise the formed formazan. The absorbance was measured at 540nm. The percentage of growth inhibition was calculated using the following formula, Values of absorbance were converted into percentage of residual viability. Usually the Inhibition concentration 50% (IC50) is chosen as the best biological marker of cytotoxicity. The IC50 value represents the concentration of the test extracts that reduced 50% of cell inhibition. Statistical analysis Statistical evaluation of data was done by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test on viability using computer based fitting program (Prism graph pad prism version 6.03) statistical significance was set at p,0.05. IC50 was calculated by linear interpolation method using the formula IC50 = Ãâ€"(D-C)+C Where A = The first point on the curve, expressed in percent inhibition, that is less than 50% B = The first point on the curve, expressed in percent inhibition, that is greater than or equal to 50% C = The concentration of inhibitor that gives A% inhibition D = The concentration of inhibitor that gives B % inhibition

The Human Genome Project Essay -- Science Scientific Research Papers

The Human Genome Project Introduction The Human Genome Project (HGP) is a plan to develop a detailed genetic and physical map of the human genome. As a result of this project, it is predicted that vast increases in technology and biological approaches to the molecular world will occur. It was speculated that this project would take two decades. Some scientist did not even think it could be done because the technology was not ready for this kind of project. Multiple issues have arisen since the original concept for this genome project was introduced. Never before had mankind faced such issues in one project. These issues include such topics as biotechnology, ethical dilemmas, social and individual implications, health and medical care, and scientific research. When the project was started in 1990, technology could only sequence 300 base pairs at a time. This made mapping (location of genes) and sequencing (the number and pattern of "letters") of the entire 3 billion base pairs seems like an insurmountable task (Collins & Jegalian, 1999). Limited technology drew critics of the project with doubt that a complete map could be accomplished. Development of technology proved to be the least controversial issue. Ethical issues often became the center of concern. Also, the fear of attempting to clone a genetically superior race became a key issue. Many of the other issues stemmed from ethical concerns. Health and medical care implications could have a profound effect on society and individuals through prevention, diagnosis, and treatment of genetic diseases. Thanks to the HGP things like insight into basic biology, development of new technology, and medicine may be increased drastically. History The official start of t... ...Human Genome Project: where did it come from, where is it going? American Journal of Human Genetics, 51, 1-6. - Spengler, S. (1997). Emerging technologies from the Human Genome Project for understanding susceptibility and risk. Environmental Toxicology and Pharmacology, 4, 235-238 - van Ommen, G., Bakker, E., & den Dunnen, J. (1999). The human genome project and the future of diagnostics, treatment, and prevention. The Lancet, 354 (suppl 1), 5-10. - Young, D. & Leonard, D. (1999). Issues in genetic testing. Clinical Chemistry, 45(6), 915-926. Websites - http://www.cnn.com/SPECIALS/2000/genome/ - http://www.celera.com/ - http://www.nih.gov/ - http://www.nhgri.nih.gov/NEWS/about_HGP.html - http://www.nhgri.nih.gov/HGP/ - http://www.ornl.gov/TechResources/Human_Genome/home.html - http://www.sciencemag.org

The Rise and Fall of ABC Learning

Q1: The aspects of the business strategy of ABC Learning resulted in increased business risk for the company including: the rapid expansion of market share, over-indebt, and blinding overseas investment. Rapid expansion of market share: ABC, which at its peak had almost 2200 centres in four countries, also had a flawed strategy to handle significant and rapid growth. When A. B. C. Learning Centers listed on the stock exchange in March 2001, it was a tiny operation with a market capitalization of just $25m. But five years later that number is approaching $2. bn as the company has quickly become Australia's leading operator of childcare centers. ABC pursued acquisition after acquisition – buying up as many existing centers as they could, and expanding their appetite by establishing more and more sites off the back of increasing debt. The company's acquisitions are getting larger so there is always a risk with this strategy that they will pay too much for a business or be unable to integrate it effectively. This meant that every new ABC sign that appeared on the horizon – equated to more and more debt. Ultimately ABC could no longer sustain their rapid expansion. With a falling share price and closer examination of their books it became clear ABC’s true value was significantly lower than previously thought. Over-indebted: In 2005, in order to satisfy the expansion plan, raising capital for domestically and globally expansion was done through issuing shares to public. ABC borrowed an enormous amount of money from Australian big four banks: CBA, NAB, Westpac and ANZ). In the wake of the global financial crisis, it couldn’t refinance its huge debts, so the administrators were called in. In the end, ABC got too big for its own good, also made itself to the end. Blinding oversea investment: After becoming the dominant player in the domestic market, ABC Learning has pursued an aggressive overseas expansion. The high levels of debt and dilutive capital raisings that have been required to fund its international ambitions have not pleased investors, and doubts about the company’s ability to repeat its local achievements in the US market have weighed on the share price. As the case told, artificially create apparent shareholder value may be misleading to potential investors in the company. Q2: Intangible assets are defined as identifiable non-monetary assets that cannot be seen, touched or physically measured, which are created through time and/or effort and that are identifiable as a separate asset. There are two primary forms of intangibles – legal intangibles (such as trade secrets, copyrights, patents, trademarks, and goodwill) and competitive intangibles (such as knowledge activities, collaboration activities, leverage activities, and structural activities). Legal intangibles are known under the generic term intellectual property and generate legal property rights defensible in a court of law. Competitive intangibles, whilst legally non-ownable, directly impact effectiveness, productivity, wastage, and opportunity costs within an organization – and therefore costs, revenues, customer service, satisfaction, market value, and share price. ABC Learning valuated billions of dollars worth of now discredited intangible assets that made up most of ABC’s balance sheet. It increased profits rapidly through acquisitions, and cause the underlying problem when valuated the assets it acquired. Especially given that 70 per cent of its assets were intangibles. The inherent risk associated with the valuation of the assets was enormous and should haven been a red flag,’ said Dr Ross. In other words, it means that ABC did not have a particularly strong balance sheet. The company lists total assets of $4. 5 billion – of which, more than $3 billion relate to intangible assets (which are predominantly child-care licences and a small amount of goodwill). As a result, ABC has negative net tangible assets. Q3: Principle-based: Accounting standards may take the form of general principles, relying on interpretation and judgment by the financial statement preparers before they can be implemented. Historical cost depreciation provides a better example of a principles-only standard. Whereas, Rule-based: Alternatively, standards may take the form of a series of rules, limiting the flexibility and use of judgment allowed in their implementation. Rules-based standards often provide â€Å"bright-lines† tests which can easily be avoided. As a result, representational faithfulness may be avoided and a low degree of comparability will often result. Numerous exceptions may also result. The advantage of principle-based accounting standard is potentially very flexible with those new and changing products and environments. As such, they should also require less maintenance. For this case, applying principle-based accounting standard would be more flexible with changing conditions, and the trade-off for this flexibility is that strong enforcement is needed to keep the auditors honest; the accountants should be more latitude to address unique situations, and it may reduce manipulation of the rules as it provides financial statements which reflect much closer to the firm’s actual performance. While the rule-based accounting standard may include a lack of flexibility, hence require almost continual maintenance at times. Therefore, the fundamentally change from â€Å"bright-line† rules-based accounting standards to principles-based accounting standards help prevent another ABC-like fiasco. The dangers in removing â€Å"bright-line rules† describes as follows: it is more difficult to audit relative to compliance, and concern over consistent and reliable interpretations across entities. In this case, the system may be less regulated, and to the extent that they rely on individual judgment to interpret and implement the standards, there is a danger that they can be used to manipulate financial results. Q4 Agency cost of debt refers to an increase in cost of debt when the interests of shareholders and management diverge. In this case, the relevant agency cost that lenders face may include large dividend payments that result in less money in the bank for loan repayment and new debt competes with old debt for repayment. Because the lack of symmetry information desires between the management of ABC learning and lenders, managers intended to maximize their personal wealth which may mean lenders’ welfare is not maximized. Based on the hypotheses that the higher the debt equity ratio the more likely managers are to use accounting methods that increase income, managers of ABC Learning may violate debt arrangements by manipulating equity. Moreover, the lenders are likely to face risk shifting in this case. Therefore, agency cost happens when ABC Learning engages in behaviors that benefit more than lenders. For lenders, they could minimize the agency cost in shortening debt maturity, it can reduces the agency cost of borrowing in two ways. First, the increase in equity value from increasing the risk of he firm's assets is a decreasing function of debt maturity (Barnea, Haugen, and Senbet, 1980). Second, shortening the maturity of debt reduces the likelihood that a firm will have to exercise an option to invest before outstanding debt matures (Myers, 1977). They also can set up a debt covenant to mitigate the risk, and from a lender’s perspective, not only does a covenant reduce default risk but is also mitigates the debt-equity agency cost. Often, individual stockholders as owners of a corporation do not have direct control over the agency contract, but as stockholders they do have certain rights granted to them. Q5: It seems that previous and current auditors had divergent opinions about the company. The new audit team from Ernst ; Young took a very different view from ABC’s previous auditors from Pitcher Partners in several aspects such as the treatment of revenues and earnings, the valuation of intangible assets, for example, after taking over the Pitcher Partners’ place, Ernst ; Young did not allow ABC to place the same high value on the licenses to run childcare centers as Pitcher Partners had done. Ernst ; Young disputed the Pitcher Partners work and the situation escalated to the extent that the ABC board was forced to call in another accounting firm, KPMG, for its opinion. One of the reasons for divergent opinions of auditors is the accounting policy choice, The accounting policy choice research area investigates the inter-relations among the contracts existing between various stakeholders of the firm, the associated economic incentives of the contracting parties, and the consequent accounting choices made by managers to influence the payoffs to the various contracting parties. The other reason is the degrees of concern of the company are different, in other words, the extent of knowledge for the auditors are different, Pitcher Partners did not have a deep knowledge of the Full Operational Status of the company, so they can not valuate the objective value on the childcare licenses. The recommendations of CLERP 9 to promote auditor independence as follows: Non-audit consultancy income for auditors has been limited and must be clearly disclosed. Auditors must provide their firms with a declaration that they are free from any relationship that may interfere with their independence. And the audit partner must rotate off a company’s audit after 5 years( or 7 years in the case of small or rural firms). Reference list: Accounting Policy Choice, viewed at 10/05/2010. http://www.business.uq.edu.au/display/research/Accounting+Policy+Choice Adam Schwab, ABC learning the hard way as profits  slump, 26 February 2008, viewed at 10/05/2010. http://www.crikey.com.au/2008/02/26/abc-learning-the-hard-way-as-profits-slump/ Ken L, John H, John S, Jennie R, 2009, Company accounting, 8th edition, John Wiley; Sons, Australian,p149. Jeremy Sammut ; Gaurav Sodhi, The ABC of child care policy failure, viewed at 9/05/2010. http://www.cis.org.au/executive_highlights/EH2008/eh71308.html John C. Easterwood , Palani-Rajan Kadapakkam, Agency conflicts, issue costs, and debt maturity, viewed at 10/05/2010. http://www.questia.com/googleScholar.qst;jsessionid=LnzP1BQzKTGJxN2BX6hnvcHzdJcSYW2TGPQNtZLgNtR20dwgMfgc!783601474!-129296667?docId=5000275458 Natasha Bita ; Andre Fraser, Imploding as easy as ABC, November 15, 2008, viewed at 10/05/2010. http://www.theaustralian.com.au/news/imploding-as-easy-as-abc/story-e6frgaio-1111118041306 Tim Searles, Not so easy at ABC, 3 Mar 08, viewed at 9/05/2010. http://www.intelligentinvestor.com.au/articles/A-B-C-Learning-Centres-Limited-ABS/Not-so-easy-at-ABC.cfm?articleID=391596 Understand The Difference Between Principle-based Versus Rule-based Accounting Standards, November 10th, 2006, viewed at 10/05/2010.

The History of Color Television

The History of Color Television The earliest mention of color television was in a 1904 German patent for  a color television system. In 1925, Russian inventor Vladimir K.  Zworykin  also filed a patent disclosure for an all-electronic color television system. While both of these designs were not successful, they were the first documented proposals for color television. Sometime between 1946 and 1950, the research staff of RCA Laboratories invented  the worlds first electronic,  color television system.  A successful color television system based on a system designed by RCA began commercial broadcasting on December 17, 1953. RCA vs. CBS But before RCA, CBS researchers led by Peter Goldmark invented a mechanical color television system based on the 1928 designs of  John Logie Baird. The FCC authorized CBSs color television technology as the national standard in October of 1950.  However, the system at the time was bulky, picture quality was terrible, and the technology was not compatible with earlier black-and-white sets. CBS  began  color broadcasting on five east coast stations in June of 1951. However, RCA responded by suing to stop the public broadcasting of CBS-based systems. Making matters worse was that there were already 10.5 million black-and-white televisions (half RCA sets) that had been sold to the public and very few color sets. Color television production was also halted during the Korean War. With the many challenges, the CBS system failed. Those factors provided RCA with the time to design a better color television, which they based on Alfred Schroeders 1947 patent application for a technology called shadow mask CRT. Their system passed FCC approval in late 1953 and sales of RCA color televisions began in 1954. A Brief Timeline of Color Television Early color telecasts could be preserved only on the black-and-white  kinescope  process introduced in 1947.In 1956, NBC began using color film to time-delay and preserve some of its live color telecasts.  A company named Ampex  made a color videotape recorder in 1958 and  NBC used it to tape  An Evening With Fred Astaire,  the oldest surviving network color videotape.In 1958, President Dwight D. Eisenhower visited the NBC station in Washington, D.C. and gave a speech discussing the new technologys merits. His speech was recorded in color, and a copy of this videotape was given to the Library of Congress.NBC  made the first coast-to-coast color broadcast when it telecast the  Tournament of Roses Parade  on January 1, 1954.The  premiere of Walt Disneys Wonderful World of Color in  September  1961 created a turning point that persuaded consumers to go out and purchase color televisions.  Television broadcasting  stations and networks in most parts of the w orld upgraded from black-and-white TVs to color transmission in the 1960s and 1970s. By 1979, even the last of these had converted to color, and by the early 1980s, black-and-white sets were mostly small portable sets  or those used as video monitor screens in lower-cost consumer equipment. By the late 1980s, even these areas switched to color sets.

Annotated Reference List Example

Annotated Reference List Example Annotated Reference List – Article Example Annotated Reference List Bendeman, Hanneli. "Alternative Dispute Resolution (ADR) in the workplace - the South African experience". African Journal on Conflict Resolution 7, no. 1 (2007): 137-161The journal takes a look at how South Africa is embracing the path of Alternative Dispute Resolution. According to this journal, the way out is to continue exploring the possibility of all alternatives that can dispense justice to all. Labor courts are used for resolving serious issues like unfair dismissal, strikes, and discrimination at workplaces.Conflict Resolution Quarterly 31, no. 4: (2014): 357-386. The journal talks about the processes of resolving conflict at workplace. The processes have permeated many organizations in North America for the past thirty years. According to the author, arbitration and mediation processes were the first ones to be used in organizations. This article takes a look at the trends in workplace conflict management and the ADR education and proposes where the field should be in the coming years. Chambliss, Daniel F. and Russell K. Schutt. Making Sense of the Social World: Methods of Investigation. Los Angeles: Sage, 2003. The book is a student-friendly and engaging introduction to the social research for students. It gives a balanced coverage of qualitative and quantitative methods, providing substantive examples and some research techniques. It covers all essential elements of research methods, including causation, validity, and techniques of analysis. Cloke, K., and Goldsmith, J. Resolving Conflicts at Work. San Francisco: Jossey-Bass, 2000. Cloke and Goldsmith state that every conflict at workplace produces an emotional response. However, most workplaces as well organizational cultures require the workers to check their emotions when they enter their workplace or leave the emotions at home. According to this book, workers can temporarily hold back their emotions, but they cannot eliminate them completely. Coates, Mary Lou, Gary T. Fu rlong, and Bryan M. Downie. Conflict management and dispute resolution systems in Canadian nonunionized organizations. Kingston, Ont: IRC Press, 1997. The purpose of this book is to develop a better understanding of the conflict management and dispute resolutions in the nonunionized workplaces in Canada. It tries to examine the role of conflict management and dispute resolution. It also takes a look at how employers are managing and resolving conflicts within their organizations.Colvin, Alexander J.S. "American workplace dispute resolution in the individual rights era". The International Journal of Human Resource Management 23, no. 3 (2012): 459-475. This article gives a theoretical conceptualization of the increase of alternative dispute resolution and the impact it has on the American employment relations in the individual rights era. It further examines the question of whether the new individual rights employment rights-based system has replaced the old one.Dickinson, David. Alte rnative dispute resolution. IZA World of Labor, (September 2014): 71. The article talks about the alternative dispute resolution procedures. It produces procedures such as mediation and arbitration as the best ways of resolving contract, wage, and grievance disputes. However, the procedures lead to changing levels of success and acceptability of the results depending on their design.References Bendeman, Hanneli. "Alternative Dispute Resolution (ADR) in the workplace - the South African experience". African Journal on Conflict Resolution 7, no. 1 (2007): 137-161.Conflict Resolution Quarterly 31, no. 4: (2014): 357-386. Chambliss, Daniel F. and Russell K. Schutt. Making Sense of the Social World: Methods of Investigation. Los Angeles: Sage, 2003. Cloke, K., and Goldsmith, J. Resolving Conflicts at Work. San Francisco: Jossey-Bass, 2000.Coates, Mary Lou, Gary T. Furlong, and Bryan M. Downie. Conflict management and dispute resolution systems in Canadian nonunionized organizations. King ston, Ont: IRC Press, 1997. Colvin, Alexander J.S. "American workplace dispute resolution in the individual rights era". The International Journal of Human Resource Management 23, no. 3 (2012): 459-475.Dickinson, David. Alternative dispute resolution. IZA World of Labor, (September 2014): 71.

Organic Food Essay Example | Topics and Well Written Essays - 1250 words

Organic Food - Essay Example The consumption of organic food has increased owing to the fact that people consider it to have a better taste. It is also believed to be environment friendly owing to the lack of chemicals used in its plantation. Studies have analyzed the fact that organic food consist of a greater amount of anti-oxidants in comparison to the conventional foods. It has also been proved that organic foods do not have traces of chemicals on them. Organic foods have a few drawbacks as well. These include the fact that they rot at a fast pace owing to lack of usage of preservative chemicals. They are also expensive in comparison to the conventional foods. The break out of salmonella disease in the United States owing to the consumption of organic peanuts also raised doubts about the credibility of organic foods. Studies have also claimed regarding the lack of strong benefits of organic foods. Despite of this, further studies and researches have proved that organic foods are beneficial. It is believed th at to prevent disease with organic foods, regulatory bodies should be made strict to check the production of these foods. Organic Food Every individual in today’s world has become health conscious and is aware of the fact that healthy eating and lifestyle changes can result in the promotion of good health. This has led to the encouragement of food choices that serve to enhance the health status of an individual. Organic food has been an essential part of this health conscious drive. According to the Department of Agriculture in the United States, organic food is that food which â€Å"is generally free of synthetic substances; contains no antibiotics and hormones; has not been irradiated or fertilized with sewage sludge; was raised without the use of most conventional pesticides; and contains no genetically modified ingredients.† The organic food was officially recognized and permitted in the United States in the year 2002. This food was originally classified to be more nutritious and healthy for the consumers. But researches were conducted to rebut this claim and to signify the fact that there is no added benefit by the consumption of these foods (Bittman 2009; Severson et al 2009). The subject of organic food has been central to many arguments as there are claims by the producers of these foods that they are beneficial for the health of the people but this has been strongly disagreed upon by a few researchers. The organic food industry has been booming in the United States at a very fast pace. It has been seen that the sales of the organic foods has increased from 11 billion dollars in 2001 to 20 billion dollars in the year 2009. In the year 2006 alone 16.7 billion dollars were achieved from the selling of organic drinks and edibles. Furthermore, a survey conducted by Harris in the year 2007 highlighted the fact that approximately 30 percent of Americans purchase and consume organic food at some point. The survey further elaborated the fact that it was a strong belief of most of the Americans that organic food is better than the conventional food. The American first lady, Michelle Obama also created an initiative for the promotion of organic foods. She started a small plantation of organic foods in the White House to support the increased usage of organic foods for the promotion of healthy eating habits amongst the Americans (Bittman 2009; Severson et al 2009). Organic food has become a top choice owing to many factors. Many people prefer to consume organic food owing to the consideration that organic food is of a greater nutritive value in comparison to the non-organic food. The restricted use of pesticides for organic foods is also considered to be an added point. This is owing to the fact that pesticides are used for the

Mugwumps Essay Example | Topics and Well Written Essays - 500 words

Mugwumps - Essay Example There were massive amounts of government corruption during the Grant administration and the resulting problems drove a wedge in the Republican Party, the Mugwumps supported Democratic reform candidates such as Grover Cleveland. According to Gerald W. McFarland, â€Å"Between 1870 and 1896, the Mugwumps, most of whom were members of the Republican party’s reform faction, frequently engaged in protests against â€Å"unsuitable† party candidates† (McFarland 40). These protests helped bolster the chances of those candidates running on a reform platform and partially assisted in the election of Grover Cleveland. However, the decision to go against ones political party was not taken lightly, as Allan Peskin writes, â€Å"To be a Republican or to be a Democrat-these were not lightly-made decisions but were shaped by self-interest, ethnicity, and, above all, history, most notably the polarizing trauma of the Civil War† (Peskin 703). As a result of the election fraud and other types of corruption that caused the backlash and mugwumps to begin their attempts at reform. The infamous Tammany Society was extremely influential during this time period as well, primarily in New York which following the Civil War had an inordinate amount of influence on national elections and politics. This was in no small part due the large influx of immigrants through Ellis Island. According to Peter Argersinger, â€Å"The highly competitive political system of the late nineteenth century, as Samuel T. McSeveny has written, â€Å"led to repeated charges, countercharges, and denials of corruption, coercion, bribery, and fraud-allegations that have been echoed by subsequent political biographers and historians. Taken at face value, these outcries would lead one to believe that the two parties alternated in cheating their opponents out of deserved victories† (Argersinger 669) It is no small wonder then that so many individuals